2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-N0667S2
    L-Asparagine-15N2 monohydrate
    		≥98.0%
    L-Asparagine-15N2 monohydrate is the 15N-labeled L-Asparagine monohydrate (HY-W017443). L-Asparagine monohydrate is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine monohydrate can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.
    L-Asparagine-<sup>15</sup>N<sub>2</sub> monohydrate
  • HY-160099
    20-5,14-HEDE
    		98.34%
    20-5,14-HEDE (WIT003) is an analog of 20-HETE. 20-5,14-HEDE activates PI3K/Akt signaling pathway, thereby exhibiting anti-apoptotic and cell survival promoting effects. 20-5,14-HEDE is the agonist for 20-HETE that increases intracellular Ca2+ concentrations, thereby enhancing vasoconstriction.
    20-5,14-HEDE
  • HY-E70090
    T7 RNA polymerase
    T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter.
    T7 RNA polymerase
  • HY-W028350
    NSC727447
    		Inhibitor
    NSC727447 is an inhibitor of ribonuclease H (Rnase H) of HIV-1 and HIV-2. NSC727447 has little activity against E. coli RNase H, but great selectivity over human Rnase H, with IC50s value of 2.0 μM, 2.5 μM, 100 μM, 10.6 μM, respectively.
    NSC727447
  • HY-N1150R
    Thymidine (Standard)
    		Inhibitor
    Thymidine (Standard) is the analytical standard of Thymidine. This product is intended for research and analytical applications. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication.
    Thymidine (Standard)
  • HY-W006102
    Bz-rA Phosphoramidite
    		99.03%
    Bz-rA Phosphoramidite is used for ribonucleotides modification.
    Bz-rA Phosphoramidite
  • HY-15620
    Methylproamine
    		Modulator 98.90%
    Methylproamine is a DNA-binding radioprotector, acts by repair of transient radiation-induced oxidative species on DNA. Methylproamine also protects against ionizing radiation by preventing DNA double-strand breaks.
    Methylproamine
  • HY-19387
    Didox
    		Inhibitor 98.24%
    Didox (NSC-324360) is a synthetic ribonucleotide reductase (RR) inhibitor.
    Didox
  • HY-100538
    DTP3
    		Inhibitor 99.56%
    DTP3 TFA is a potent and selective GADD45β/MKK7 inhibitor. DTP3 TFA targets an essential, cancer-selective cell-survival module downstream of the NF-κB pathway.
    DTP3
  • HY-B1050
    Gemifloxacin mesylate
    		Inhibitor 99.76%
    Gemifloxacin mesylate (SB-265805S; LB-20304a) is an orally active broad-spectrum quinolone antibacterial antibiotic. Gemifloxacin mesylate inhibits DNA synthesis by inhibiting DNA gyrase and Topoisomerase IV activities. Gemifloxacin mesylate has potent antibacterial activities against gram-positive bacteria in vitro efficacy study, particularly Streptococci and Staphylococci. Gemifloxacin mesylate has been used in the research of respiratory tract infections.
    Gemifloxacin mesylate
  • HY-156579
    WRN inhibitor 3
    		Inhibitor 99.54%
    WRN inhibitor 3 (example 110), a cyclic vinyl sulfone compound, is a Wemer Syndrome ATP dependent helicase enzyme (WRN) inhibitor. WRN inhibitor 3 can be used for the research of cancer.
    WRN inhibitor 3
  • HY-162471
    GSK_WRN3
    		Inhibitor 98.54%
    GSK_WRN3 is selective WRN helicase inhibitor (pIC50 = 8.6). SK_WRN3 can selectively inhibit the growth of microsatellite unstable (MSI) cancer cells, induce DNA damage, and cause cell cycle arrest. GSK_WRN3 has anti-tumor activity.
    GSK_WRN3
  • HY-145056
    ART615
    		Control 98.65%
    ART615 is the related isomer of ART558. ART615, the inactive of ART558, elicits <10% Polθ inhibition at 12 µM, thus serving as a control for ART558 (IC50=7.9 nM).
    ART615
  • HY-131123
    N-Nitrosomorpholine
    		Inhibitor 99.91%
    N-Nitrosomorpholine is a photosensitive nitrosamine that is carcinogenic and can be used to construct animal cancer models.
    N-Nitrosomorpholine
  • HY-121862
    CM03
    		Inhibitor 99.73%
    CM03 is a potent DNA G-quadruplexes (G4s) ligand. CM03 can stabilise G4s, downregulating more G4-containing genes as well as increasing incidence of double-strand break events (DSBs) due to torsional strain on DNA and chromatin structure. CM03 has selective potency for pancreatic cancer cells.
    CM03
  • HY-163573
    FANCM-BTR PPI-IN-1
    		98.61%
    FANCM-BTR PPI-IN-1 (Comp 32) is a disruptor of the FANCM/ BTR interaction which hampers the FANCM localization to telomeres. FANCM-BTR PPI-IN-1 can be used for cancer research.
    FANCM-BTR PPI-IN-1
  • HY-138597
    5'-O-TBDMS-dT
    		Activator 99.43%
    5'-O-TBDMS-dT is a nucleoside with protective and modification effects.
    5'-O-TBDMS-dT
  • HY-W020044R
    DL-alpha-Tocopherol (Standard)
    		Inhibitor 99.06%
    DL-alpha-Tocopherol (Standard) is the analytical standard of DL-alpha-Tocopherol. This product is intended for research and analytical applications. DL-alpha-Tocopherol is a synthetic vitamin E, with antioxidation effect. DL-alpha-Tocopherol protects human skin fibroblasts against the cytotoxic effect of UVB.
    DL-alpha-Tocopherol (Standard)
  • HY-N1377
    Nevadensin
    		Inhibitor 99.93%
    Nevadensin (Lysionotin), a natural flavonoid, is a selective human carboxylesterase 1 (hCE1) inhibitor with an IC50 of 2.64 μM. Nevadensin is more selective for hCE1 than hCE2 (IC50 of 132.8 μM). Nevadensin can induce apoptosis and DNA damage in cancer cells. Nevadensin has a variety of pharmacological effects such as anti-inflammatory, anti-tumour, anti-hypertensive, anti-tubercular, antitussive, antioxidant and anti-microbial activities.
    Nevadensin
  • HY-W013514
    2-Acetamidofluorene
    		Inhibitor 98.83%
    2-Acetamidofluorene is a potent carcinogenan. 2-Acetamidofluorene is can be used fot induction of hepatocellular carcinoma (HCC) and multiple primary tumours.
    2-Acetamidofluorene
Cat. No. Product Name / Synonyms Application Reactivity